Puneet juneja
@chattbuzzStructural Biochemist @Thermo Fisher @Novavax
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The @CryoEM_ISU at @IowaStateU has an open position for a Research Scientist I to support ongoing research activities in Cryo-EM to support academic and industrial partners. For more job details use the application link: isu.wd1.myworkdayjobs.com/IowaStateJobs/…
Hi #cryoEM! @Nayimcete and I have made some tools that we find quite useful for cryoEM data processing at different stages. Have a look. We hope you find them useful! If you feel like contributing, please go ahead. That would be awesome! Any feedback would be very welcomed too!
Excited to discuss our new preprint that has been a work in progress for the past two years! TL;DR – we propose a new framework for #cryoEM data collection that makes decisions and plans a path across cryo-EM grids biorxiv.org/content/10.110… Thread 👇
And for our second #CRISPR preprint of the day: The first cryo-EM structures from the @CryoEM_ISU facility! Yukti @yukti__dhingra, Shravanti @shra_krishna, and Puneet @chattbuzz report cryo-EM structures and biochemistry of the type I-C Cas4-Cas1-Cas2 complex! Thread...
PAM binding ensures orientational integration during Cas4-Cas1-Cas2 mediated CRISPR adaptation biorxiv.org/cgi/content/sh… #bioRxiv
Happy to report this Cas4 story with @shra_krishna and @sashital demonstrating how Cas4-Cas1-Cas2 updates the CRISPR array with new spacers in type I-C systems! Been an exciting journey towards the first cryoEM story from @CryoEM_ISU and working alongside Puneet @chattbuzz
And for our second #CRISPR preprint of the day: The first cryo-EM structures from the @CryoEM_ISU facility! Yukti @yukti__dhingra, Shravanti @shra_krishna, and Puneet @chattbuzz report cryo-EM structures and biochemistry of the type I-C Cas4-Cas1-Cas2 complex! Thread...
We are expanding the @UWBiochem CEMRC and the #NIH National CryoET Center! Interested in #CryoEM, #teamtomo, #CLEM, and working with a great #microscopy team? Check out the scientist advert! | Also great #Postdoc posts | DM & RT jobs.hr.wisc.edu/en-us/job/5110…
My 3 years old asking questions about work and correctly identified protein as POPCORN.
Looking forward to this one: always good fun. Not a prof though. 🤪
Join @SBGRid fall webinar series. Starting next week: GROMACS, RELION, MoRDa, PyMOL & talks from Niko Grigorieff's, Yi-Wei Chang's, and Alexander Sobolevsky's groups. sbgrid.org With @GMX_TWEET @Schrodinger @SjorsScheres, @AlexanderSobol6, @yiweichang et al.
Screening #cryoEM grids at the @IowaStateU CryoEM Facility 🧊🔬 #UIowaResearch @PCF_uiowa @IowaMed
Online now: "No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing" - Read the Report from @Faulkner_Lab and colleagues in @CellReports hubs.ly/H0TDHYM0
Manuscripts are assessed the same no matter where the authors are from
Is this the start of the resolution revolution in AFM? #journalclub doi.org/10.1038/s41586…
Interested in working in #structuralbiology at the interface between #CryoEM & #xray ? We are expanding our team @UWBiochem Check out the advert! jobs.hr.wisc.edu/en-us/job/5093… | DM & RT
Join the eBIC team @DiamondLightSou for this practical #cryoEM course, 26-29 April: ‘Electron Cryo Microscopy in Structural Biology’ covering topics from sample prep through to image processing, reconstruction & modelling. Open to attendees from industry: loom.ly/UsnZCOk
A Further Turn of the Screw chemistrycommunity.nature.com/posts/a-furthe… A Behind the Paper story on our recent Nature Communications article @ManuelaOrdy @jerrynosnothing @conticellolab @EmoryChem
Synthetic shape-shifting collagen with potential biomedical applications - Materials Today materialstoday.com/biomaterials/n… A nice summary of our recently published research in Materials Today. @conticellolab @EmoryChem
Breaking the next Cryo-EM resolution barrier - Atomic resolution determination of proteins! biorxiv.org/cgi/content/sh…
Release the cryoDRGN ❄️🐉 (on biorxiv)! Instead of static protein structures, we develop a neural network architecture to reconstruct diverse conformations and molecular motions from real cryo-EM image data. Joint work with Tristan Bepler, Bonnie Berger, and @jhdavislab
CryoDRGN: Reconstruction of heterogeneous structures from cryo-electron micrographs using neural networks biorxiv.org/cgi/content/sh…
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