Lucien Hinderling
@lhinderlingtries to make microscopes smarter · bioimage analysis, optogenetics, ml, 3d printing, open science · phd student in cellular signalling dynamics @Pertzl
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Real-time segmentation of fibroblasts allows us to grow actin skeleton in a specified direction! Cells built @Pertzlab express optogenetic construct to activate RAC. We use @manager_micro and @napari_imaging to train a pixel classifier for segmentation on live data from scope. 🧵
Playing a bit with #inTRACKtive in #CellTracksColab and it works so beautifully well!😍 Thanks to @TeunHuijben and @loicaroyer for the tool 🙏
Gamechanger for vesicle Control! We present an approach to remotely control endosomal distribution in living cells (even in #3Dmatrix) with high precision. #Magnetogenetics meets #Rabs! biorxiv.org/content/10.110… @integrintraffic @wtccmr @Gregor_lab @imgprague @mcoppey #CellBiology
Next to our fancy Leica microscope live the timelapse chambers I built for ~$150, using old microscopes and cameras that were gathering dust in the basement. The synchronization of lights, camera, focus, and watering make them more useful than the Leica for long-term imaging :)
can't believe this is working out
Wrapping neurites with small light-controlled polymer patches 😮 nature.com/articles/s4200…
Want to learn how to use microSAM for annotating your data and missed our live workshop at #i2k ? You can join for the I2K virtual seminar on Monday where @AnwaiArchit will present our tool again: tinyurl.com/Virtual-I2K202…
It can also make "uncoil" animations [like this one x.com/katpyxa/status…] (2/N)
still learning how to explore these large microsopy datasets. new trick: letting claude write small interactive UIs, customized to our data. happy to hear your tips!!
Thermal expansion -> a typical problem in time-lapse microscopy at high resolution. Here I measure the axial shift of the focal plane of a Nikon 40x0.95 air objective at ~1.6um/K, or about ~1.5x the depth of field per degree C: github.com/amsikking/Niko…
We are building a new bioimage analysis platform at the University of Bern! To setup and develop the platform we are hiring for three years an IT specialist and a Bioimage Analyst. All the infos here: ohws.prospective.ch/public/v1/jobs… and ohws.prospective.ch/public/v1/jobs…
“Hello World” may seem simple, but it symbolizes a programmer's first command over a machine, unlocking endless possibilities. 🪰✍️ Now, fruit flies echo this milestone by writing 'Hello World' with their walking paths. (biorxiv.org/content/10.110…) #Robotics #Drosophila
Thanks for reading us ! And reporting on our work 😆
New @preLights alert ✨ ARHGEF17’s new role in actomyosin collapse!! Covering recent @biorxiv_cellbio preprint from @PertzL team. prelights.biologists.com/highlights/fee…
Take a look at Convpaint ! Super easy segmentation of a wide variety of images ! Directly in the Napari ecosystem !
Huge update to Convpaint, and preprint out on bioRxiv! We develop a pixel classifier similar to Ilastik, but built for napari & with technical improvements. Now even works on animals! 1/10
Smart microscopy: analytical feedback in real-time to increase efficiency and throughput of your imaging workflows! nature.com/articles/s4159…
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Who to follow
-
napari🔬
@napari_imaging -
FocalPlane
@focalplane_jcs -
Estibaliz Gómez de Mariscal, PhD
@gomez_mariscal -
Jennifer Waters
@JenCWaters -
Alvaro Crevenna
@AlvaroCrevenna -
Alexis Lomakin, Ph.D. (אל) אַלעקסיס לאָמאַקין
@Alexis_Lomakin -
Xavier Trepat
@XavierTrepat -
Robert Haase
@haesleinhuepf -
Spirochrome
@spirochrome -
openUC2
@OpenUc2 -
Benedict Diederich
@beniroquai -
Loïc A. Royer 💻🔬⚗️
@loicaroyer -
Ulrike Boehm 🔬👩🏻💻
@Ulrike_Boehm -
Lenne Lab
@Equipe_lenne -
Rocco D'Antuono
@RogerDAntuono
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