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Want a great tip to minimise DNA stain use for PCR and agarose gel electrophoresis to save money and resources? You could reduce use and cost by up to 99%! Here's a great article on how to do it, which stains work best with it, and what to expect 👇 The method is described in a…
Here’s a very cheap, rapid, accessible, and scalable DNA purification method suitable for viral RNA or bacterial DNA. It's been validated for use in SARS-CoV-2 (COVID-19) detection, and has more recently been used to purify DNA to allow the detection of toxin-producing E. coli in…
My best article @ScienceMagazine It took ~1 year for me to publish it since its 1st submission. I hope it will positively change the #culture. I hope you will work together with me to make a better world for the next #generations. Please retweet broadly. science.org/content/articl…
AI-generated bacterial chromosome. Someone needs to build and test this.
Evo also has the potential to generate sequences at the scale of whole genomes. We generated DNA sequences over 650 kilobase on a single GPU. When we sample sequences at this length with Evo, we find genomes that contain thousands of potential protein-coding sequences
Here’s a very simple and clever method of loading up to 800 μL of a nucleic acid solution into a single well of an electrophoresis gel, allowing the detection, concentration, and recovery of extremely dilute DNA without the need for additional equipment or reagents. The method,…
For anyone learning or teaching bioinformatics, or if you’re interested in getting started, you may like to check out some of many (>230!) integrated tutorials for the Galaxy bioinformatics platform at training.galaxyproject.org/training-mater… . The Galaxy platform is a free, easy-to-join,…
Another exciting new tool to engineer biology from the Jason Chin Lab >>> Establishing a synthetic orthogonal replication system enables accelerated evolution in E. coli | Science science.org/doi/10.1126/sc…
Nice write-up of the pAblo·pCasso #CRISPR technology developed by @KaterinaKozaeva & @ManuelNietoDom1 @LabNikel by @SynBioBeta complete with cool #AI-generated graphical displays. You can check the original @NAR_Open in the link below🦠🧬 synbiobeta.com/read/pablo-pca…
Off to a good start in 2024! Thrilled to share the pAblo·pCasso toolset for unconstrained cytidine/adenine-base editing in #bacteria led by @KaterinaKozaeva w/Zach Nielsen & @ManuelNietoDom1 @LabNikel @DTUBiosustain🦠@NAR_Open #SynBio #OpenAccess #CRISPR academic.oup.com/nar/advance-ar…
Are you a PhD student or early post-doctoral researcher interested in engineering synthetic systems? Register your interest for the upcoming practical course "Synthetic biology in action: engineering synthetic systems" by visiting: bit.ly/3SdB8pN @EMBO @embl
More people should start a blog. Writing on the internet is the most important thing I've done for my career. A well-written article is like a beacon that attracts like-minded thinkers. Here are 18 of my favorite articles about why you should write & how to do it... WHY YOU…
For anyone looking for freeware electrophoresis gel analysis software, check out GelAnalyzer 23.1 by Dr. Istvan Lazar Jr. and Dr. Istvan Lazar Sr. As you can guess from the version number this software has been around for a while (since 2010) and has been quite popular over this…
Exciting news! Did Acidobacteria also pop-up in your MAGs? In our new paper we identify sponge-type polyketides from this unusual phylum: sciencedirect.com/science/articl… Have a read for more details. We are excited to see where else we can find Acidos!
Extreme miniaturization in plasmid design: generation of the 903 bp cloning vector pICOt2 ift.tt/NQlf4Ku #biorxiv_synbio
Here is a great application of high-throughput DNA barcoding using Oxford Nanopore MinION: fighting the illegal pangolin product trade by sequencing 2346 scales at a cost of less than 1 USD per sequence! The study, by Yeo et al. (2023), is probably the first study to use…
Good education materials by @MichaelCJewett @LucksLab et al. @ACSSynBio At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…
I have failed...but I couldn't be happier. A small thread on recent experiments 🧬🔀🧵 1/n
📢Introducing GenePT, our new #gene + #singlecell #foundationmodel built from #ChatGPT. It's simple yet surprisingly effective! 1. we use text summary + #ChatGPT to generate embedding for each gene 2. cell embedding = avg of gene vectors weighted by expression GenePT can…
For anyone interested in cheap “homemade” DNA extractions using spin columns, or learning what is in most commercial kits, here’s a great modular system of extraction methods called “Mu-DNA”. Mu-DNA stands for “Modular Universal DNA extraction method", and it should be able to…
Another very clever and commonly used method of doing single-tube nested PCR is to separate the two rounds of PCR by using different annealing temperatures rather than by spatial separation of the two primer sets. This can be done by designing the “outer” primers for the first…
Here’s an interesting PCR method for working with extremely low concentrations of DNA: “Booster PCR”. It looks like a rarely used and now obsolete method for most research, but it may be worth a try to give a PCR a boost if you can't access other more sensitive amplification or…
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