@AmandaChaplin10 Profile picture

Amanda Chaplin

@AmandaChaplin10

Lecturer in DNA repair

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Amanda Chaplin Reposted

We have used AUC and cryo-EM to investigate oligomeric states of dye-decolorizing peroxidases from Streptomyces and the implications these states have for function @biowirelab @AmandaChaplin10 @LeicStructBio @EssexLifeSci 😀 onlinelibrary.wiley.com/doi/10.1002/pr…


Amanda Chaplin Reposted

Very happy to finally finish this paper and the corresponding code and tutorial. Grateful to all those who helped make this happen. Hopefully it will contribute to making #cryoET more accessible. #teamtomo biorxiv.org/content/10.110…


Amanda Chaplin Reposted

We are excited to relaunch the Midlands Biophysical Network. Sign up via the link below @uniofleicester @unibirmingham @uniofwarwick @UniofNottingham @LeicStructBio @InnovationMids

The Midlands Biophysical Network is starting up again! 17 September Midland Biophysics Meeting at Leicester. @HeskethEmma Sign up here forms.office.com/Pages/DesignPa…



Amanda Chaplin Reposted

#Endometriosis takes 7-10 years to diagnose, on average; but a surge in research is finally leading to new diagnostics and treatments. News feature from @clarewhatson #InternationalWomensDay2024 nature.com/articles/s4159…


Amanda Chaplin Reposted

I don't want to beat our own drum here, but I just wanted to say that #ModelAngelo by @jamaliki1998 is a fantastic program that has yet again completely blown me away today. 🥳


Amanda Chaplin Reposted

Our article elucidating the structural role of Lig4 in DNA end synapsis during NHEJ is now out at Nat Comms link.springer.com/10.1038/s41467…


Amanda Chaplin Reposted

Our new collaborative manuscript with @SebBritton1 and Calsou lab identifying a structural role for DNA-PKcs in regulating Ku stiochiometry during DSB repair. biorxiv.org/content/10.110…


Amanda Chaplin Reposted

Structural and functional basis of inositol hexaphosphate stimulation of NHEJ through stabilization of Ku-XLF interaction by @AmandaChaplin10 in @NAR_Open pubmed.ncbi.nlm.nih.gov/37870477/

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Amanda Chaplin Reposted

POLQ and Pol Delta are working together to synthesize DNA during DSB repair via MMEJ. 👏👏👏 Also explains how POLQ seals post-replicative ssDNA gaps on the lagging strand during replication, which I and others have previously observed. nature.com/articles/s4158…


Amanda Chaplin Reposted

Our paper is out, Bravo and thanks to all the contributors!!👏👏 Take home: after DSB induction, gH2AX domains self-segregate to form a new chromatin compartment, in which some DDR genes also physically relocate to potentiate their expression. rdcu.be/doT6W


Amanda Chaplin Reposted

Head over to our blog to read about last month's 2023 Lister Institute Annual Meeting, where our community gathered for an inspiring two days of talks at Corpus Christi, Oxford. #ListerFellows #science lister-institute.org.uk/lister-annual-…


Amanda Chaplin Reposted

My new review on the mechanism of NHEJ is out in DNA Repair. It provides the emerging view of how DNA end synapsis and processing are coordinated. authors.elsevier.com/sd/article/S15…


Amanda Chaplin Reposted

[🗓️ #event] 🧬Interested by #NHEJ pathway and its roles in development and #cancer ? It's still time to register for the three day international meeting that will be held in Fontenay-aux-Roses (France 🇫🇷) on September 27-29, 2023. All informations online➡️nhej2023.sciencesconf.org

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Amanda Chaplin Reposted

So nice collaboration with Amanda Chaplin, Patrick Calsou and Sébastien Britton. Congrats to He's lab in back to back

Check-out our new publication in @ScienceAdvances where we decipher at the structural and functional levels how PAXX and XLF can interact simultaneously with Ku to promote DNA end synapsis and proficient #NHEJ #DNA_repair doi.org/10.1126/sciadv…

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Amanda Chaplin Reposted

Great structures of Ku-PAXX made by Muriel from @CharbonnierJea7 lab and collaborators from @AmandaChaplin10 lab published in @ScienceAdvances doi.org/10.1126/sciadv…. Congratulations 🎉!

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Amanda Chaplin Reposted

Also check out the related research article from @YuanHe_NWU science.org/doi/10.1126/sc…


Amanda Chaplin Reposted

This was part of a highly collaborative project including ourselves at @BiocEMcam, @CharbonnierJea7 , Kathy Meek, Patrick Calsou and others


Amanda Chaplin Reposted

The latest work from @AmandaChaplin10, work initiated at @CamBiochem and continued at @LeicStructBio science.org/doi/10.1126/sc…


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